Journal: Thrombosis Journal
Article Title: Procoagulant effect of phosphatidylserine-exposed blood cells, endothelial cells and extracellular vesicles in patients with aortic stenosis
doi: 10.1186/s12959-025-00755-3
Figure Lengend Snippet: The EVs in healthy controls ( n = 50), mild AS patients ( n = 55), moderate AS patients ( n = 51) and severe AS patients ( n = 60) were analysed by flow cytometry. ( A ) Alexa-Fluor 488-lactadherin binding was used to determine that EVs gated in this way were PS + . ( B , C , D ) PEVs (Alexa Fluro 647-CD41a + ), EEVs (Alexa Fluro 488-CD31 + /Alexa Fluor 647-CD41a − ) and TF + EVs (Alexa Fluor 647-CD142 + ) were also measured and counted by co-labeling with Alexa Fluor 488 − and Alexa Fluor 647-labeled antibody. PS + EVs: phosphatidylserine-positive extracellular vesicles; EEVs: endothelial-derived extracellular vesicles; PEVs: platelet derived extracellular vesicles; TF + EVs: tissue factor-positive extracellular vesicles. * p < 0.05
Article Snippet: CD142 (clone HTF-1), CD41a (clone HIP8), purified CD31 (clone L133.1) and TruCount Tube from Becton Dickinson (San Jose, CA, USA) were also used.
Techniques: Flow Cytometry, Binding Assay, Labeling, Derivative Assay